Live imaging of mitosomes and hydrogenosomes by halotag technology

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• Título: Live imaging of mitosomes and hydrogenosomes by halotag technology
• Autor: Sanin Peña, David Esteban; De Napoli, Maximiliano; Eshar, Shiri; Gualdron, Melisa; Hopp, Christine S; Martincová, Eva; Najdrová, Vladimíra; Tembo, Dumizulu; Van Tyne, Daria; Voleman, Luboš; Walker,Dawn
• Publicación original: PLoS ONE; Vol. 7, No. 4, 2012
• Descripción física: PDF
• Nota general:
  • Hydrogenosomes and mitosomes represent remarkable mitochondrial adaptations in the anaerobic parasitic protists such as Trichomonas vaginalis and Giardia intestinalis, respectively. In order to provide a tool to study these organelles in the live cells, the HaloTag was fused to G. intestinalis IscU and T. vaginalis frataxin and expressed in the mitosomes and hydrogenosomes, respectively.
    The incubation of the parasites with the fluorescent Halo-ligand resulted in highly specific organellar labeling, allowing live imaging of the organelles. With the array of available ligands the HaloTag technology offers a new tool to study the dynamics of mitochondria-related compartments as well as other cellular components in these intriguing unicellular eukaryotes.
• Notas de reproducción original: Digitalización realizada por la Biblioteca Virtual del Banco de la República (Colombia)
• Notas:
  • Resumen: Giardia intestinalis; Hidrogenosomas; Mitosomas; Protistas anaeróbicos; Trichomonas vaginalis
  • © Derechos reservados del autor
  • Colfuturo
• Forma/género: texto
• Idioma: inglés
• Institución origen: Biblioteca Virtual del Banco de la República
• Encabezamiento de materia:
  • Ciencias naturales y matemáticas; Ciencias naturales y matemáticas / Ciencias de la vida Biología; Tecnología; Tecnología / Ciencias médicas Medicina